Abstract
In vitro cultures of the liverwort, Fossombronia pusilla, have been initiated from spores. They grew well on Gamborg B5 medium supplemented
with 2% saccharose and 12.5 µg/l vitamin B12. Undifferentiated cultures were obtained on Knop mineral solution with 4% glucose.
The cultures produced the same constituents as collected material. Perrottetianal
A and B, known substances from liverworts were isolated and characterized by spectroscopic
methods as well as α-(-)-santonin. A hitherto unknown diterpene dialdehyde - 8-hydroxy-9-hydroperrottetianal
A - could also be characterized. Furthermore, 7 terpenes were identified by GC/MS.
A petrol ether extract and the isolated terpenes exerted antibacterial activity.
Key words
Fossombronia pussilla
- liverwort -
in vitro culture - diterpene dialdehydes - antimicrobial acitivity
