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Planta Med 1990; 56(2): 182-186
DOI: 10.1055/s-2006-960921
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© Georg Thieme Verlag Stuttgart · New York

Structural Characterization and Antitumor Activity of a Pectic Polysaccharide from the Roots of Angelica acutiloba 1

Haruki Yamada2 , Kanki Komiyama3 , Hiroaki Kiyohara2 , Jong-Chol Cyong2 , Yumiko Hirakawa3 , Yasuo Otsuka2
  • 2Oriental Medicine Research Center of The Kitasato Institute, 5-9-1 Shirokane, Minato-ku, Tokyo 108, Japan
  • 3The Kitasato Institute, 5-9-1, Shirokane, Minato-ku, Tokyo 108, Japan
1 Studies on Polysaccharides from A acutiloba. Part 13. For Part 12, see: (23)
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Publication History

1989

Publication Date:
05 January 2007 (online)

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ErrataPlanta Med 1990; 56(04): 420-420
DOI: 10.1055/s-2006-961001

Abstract

The polysaccharide fraction from the root of Angelica acutiloba Kitagawa showed a potent antitumor activity against ascitic form of Sarcoma-180, IMG carcinoma, and Meth A fibrosarcoma as well as the solid form of MM-46 tumor. An active polysaccharide, AR-4E-2, was purified by precipitation with cetyltrimethylammonium bromide, anion-exchange chromatography on DEAE-Sepharose, and gel filtration on Sepharose CL-4B from the polysaccharide fraction. An active polysaccharide fraction showed a weak anticomplementary activity. AR-4E-2 was composed of arabinose, galactose, and rhamnose in the molar ratios of 3.3 :1.0:0.7, and also contained 14.5% galacturonic acid and 3.2% protein. Methylation analysis and bas-catalysed β-elimination studies suggested that AR-4E-2 contained a rhamnogalacturonan moiety in which 2,4-di-substituted rhamnose residues were attached to 4-substituted galacturonic acid through position 2 of rhamnose. AR-4E-2 also contained highly branched 3,5-arabinan and (l→4)-galactan.

Key words

Antitumor activity - anticomplementary activity - pectic arabinogalactan - Angelica cutiloba Kitagawa