Planta Med 1990; 56(1): 53-58
DOI: 10.1055/s-2006-960883

© Georg Thieme Verlag Stuttgart · New York

Formation of Digitalis lanata Clone Lines by Shoot Tip Culture

Beate Diettrich, Heidelore Mertinat, Martin Luckner
  • Sektion Pharmazie der Martin-Luther-Universität Halle-Wittenberg, Weinbergweg 15, DDR-4010 Halle, German Democratic Republic
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Publication History


Publication Date:
05 January 2007 (online)


Propagation of Digitalis lanata by shoot tip culture made possible (a) the rapid multiplication of elite plants with the formation of plant clones and (b) the long-term cultivation of these plants which under normal growth conditions would die at the end of the second vegetation period. Optimum conditions were established for the regeneration of shoots from shoot tips, for daughter shoot formation and rooting as well as for the adaptation of the regenerated plants to the open ground. Gene banks of valuable clones were built by keeping shoots at 4°C on media with high sucrose concentration (maximum period of storage 2 years) or by growing juvenile clone plants in the greenhouse at temperatures preventing the induction of flowering. The clone plants were in the juvenile state even if they were derived from flowering mother plants. They showed normal growth and development.