Inhibition of Activator Protein 1 Activity by Berberine in Human Hepatoma Cells

Kazunori Fukuda; Yuko Hibiya; Michihiro Mutoh; Masatoshi Koshiji; Seigou Akao; Hisayoshi Fujiwara

doi:10.1055/s-2006-960795

Planta Medica, Inhaltsverzeichnis

Planta Med 1999; 65(4): 381-383
DOI: 10.1055/s-2006-960795

Letters

Inhibition of Activator Protein 1 Activity by Berberine in Human Hepatoma Cells

Kazunori Fukuda¹ , Yuko Hibiya² , Michihiro Mutoh² , Masatoshi Koshiji³ , Seigou Akao¹ , Hisayoshi Fujiwara³

¹Department of Oriental Medicine, Gifu University School of Medicine, Gifu, Japan
²Cancer Prevention Division, National Cancer Center Research Institute, Tokyo, Japan
³Second Department of Internal Medicine, Gifu University School of Medicine, Gifu, Japan

Abstract

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Abstract

Activator protein 1 (AP-1) is a transcription factor which plays a critical role in inflammation and carcinogenesis. The present study was conducted to investigate the effect of berberine, an isoquinoline alkaloid present in plants of the genera Berberis and Coptis, on the activity of AP-1 using a reporter gene assay in human hepatoma cells. Berberine was shown to inhibit AP-1 activity in a dose- and time-dependent manner at concentrations higher than 0.3 µM. Berberine inhibited AP-1 activity almost completely as low as 10 µM after 48 h treatment. The inhibitory effect on AP-1 activity in cancer cells may further explain the anti-tumor promoting activity of berberine.

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