Planta Med 1991; 57(2): 122-124
DOI: 10.1055/s-2006-960046

© Georg Thieme Verlag Stuttgart · New York

In Vitro Production of Essential Oil from Proliferating Shoots of Rosmarinus officinalis 1

Madhu Jain2 , R. Banerji3 , S. K. Nigam3 , J. J. C. Scheffer4 , H. C. Chaturvedi2
  • 2Tissue Culture Laboratory, National Botanical Research Institute, Lucknow-226001, India
  • 3Pharmaceutical Chemistry, National Botanical Research Institute, Lucknow-226001, India
  • 4Vakgroep Farmacognosie, Gorlaeus Laboratoria, Postbus 9502, 2300 RA Leiden, Netherlands
1 NBRI Research Publication No. 363 (N.S.)
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Publication Date:
05 January 2007 (online)


The 40-day-old in vitro proliferating shoots of Rosmarinus officinalis L. var. genuina forma erectus produced an appreciable quantity of essential oil, i.e., 1.8% fw, which was similar in its constituents to that obtained from 1-year-old plants, whether naturally grown or in vitro-raised potted plants. However, the quantity of various constituents identified so far was generally, but marginally, less in the former case than the latter two kinds of 1-year-old plants with the exception of bornyl acetate and 1,8-cineole, the concentrations of which were higher in the proliferated shoots than the plants. The essential oil content of 1-year-old naturally grown plants was 2.4% fw, while it was 2.38% fw in the in vitro-raised potted plants of the same age.