Planta Med 1994; 60(1): 30-33
DOI: 10.1055/s-2006-959402

© Georg Thieme Verlag Stuttgart · New York

Effects of Stinging Nettle Root Extracts and Their Steroidal Components on the Na+, K+-ATPase of the Benign Prostatic Hyperplasia1

Toshihiko Hirano, Masato Homma, Kitaro Oka
  • Department of Clinical Pharmacology, Tokyo College of Pharmacy, 1432-1 Horinouchi, Hachioji, Tokyo 192-03, Japan
1 Part of this work was presented at the IVth Clinical Experimental Conference Concerning Questions to the Benign Prostatic Hyperplasia, Nov. 12-15, 1992, Eze
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Publication Date:
04 January 2007 (online)


The effects of organic-solvent extracts of Urtica dioica (Urticaceae) on the Na+,K+-ATPase of the tissue of benign prostatic hyperplasia (BPH) were investigated. The membrane Na+,K+-ATPase fraction was prepared from a patient with BPH by a differential centrifugation of the tissue homogenate. The enzyme activity was inhibited by 10-4-10-5M of ouabain. The hexane extract, the ether extract, the ethyl acetate extract, and the butanol extract of the roots caused 27.6-81.5% inhibition of the enzyme activity at 0.1 mg/ml. In addition, a column extraction of stinging nettle roots using benzene as an eluent afforded efficient enzyme inhibiting activity. Steroidal components in stinging nettle roots, such as stigmast-4-en-3-one, stigmasterol, and campesterol inhibited the enzyme activity by 23.0-67.0% at concentrations ranging from 10-3-10-6M. These results suggest that some hydrophobic constituents such as steroids in the stinging nettle roots inhibited the membrane Na+,K+-ATPase activity of the prostate, which may subsequently suppress prostate-cell metabolism and growth.