Planta Med 1995; 61(1): 26-30
DOI: 10.1055/s-2006-957992

© Georg Thieme Verlag Stuttgart · New York

Inhibitory Effects of Oriental Herbal Medicines on IL-8 Induction in Lipopolysaccharide-Activated Rat Macrophages

Gyeong-Im Lee1 , Joo Young Ha1 , Kyung Rak Min1 , Hideo Nakagawa2 , Susumu Tsurufuji3 , Il-Moo Chang4 , Youngsoo Kim1
  • 1College of Pharmacy, Chungbuk National University, Cheongju 360-763, Korea
  • 2Faculty of Pharmaceutical Sciences, Toyama Medical and Pharmaceutical University, Toyama, Japan
  • 3Institute of Cytosignal Research Inc., Tokyo, Japan
  • 4Natural Products Research Institute, Seoul National University, Seoul 110-460, Korea
Further Information

Publication History



Publication Date:
04 January 2007 (online)


Cytokine-induced neutrophil chemoattractant (CINC), a rat interleukin-8 (IL-8), was quantitated by using a sensitive ELISA. The CINC was induced up to 20 ng/ml from basal 1-2 ng/ml in lipo-polysaccharide (LPS)-activated peritoneal macrophages. This CINC induction was significantly inhibited by steroidal anti-inflammatory drugs including dexamethasone, but not by non-steroidal drugs including indomethacin at all. Nine out of 59 herbal medicines which are frequently used in Korean traditional prescriptions for inflammatory diseases exhibited more than 50% of inhibition on the CINC induction by their total methanol extracts with 0.1 mg/ml as a final concentration. The active 9 total extracts were prepared from radix of Aralia continentalis, rhizoma of Cnidium officinale, rhizoma of Coptis chinensis, tuber of Fritillaria verticillata, radix of Saussurea lappa, tuber of Sparganium stoloniferum, flower of Syzygium aromaticum, semen of Trichosanthes kirilowii, and herba of Tripterygium regelii. These total extracts were sequentially fractionated with dichloromethane, ethyl acetate, butanol, and water. Among the solvent-fractionated extracts with 0.05 mg/ml as a final concentration, more than 50% of inhibition on the CINC induction was exhibited by the dichloromethane fraction of Aralia continentalis; the water fraction of Fritillaria verticillata; the dichloromethane fraction of Saussurea lappa; the dichloromethane, ethyl acetate, and butanol fractions of Syzygium aromaticum; the dichloromethane, ethyl acetate, and water fractions of Trichosanthes kirilowii; and the dichloromethane and ethyl acetate fractions of Tripterygium regelii.