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Planta Med 1996; 62(4): 336-340
DOI: 10.1055/s-2006-957897
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© Georg Thieme Verlag Stuttgart · New York

Application of MS-MS for the Rapid, Comparative Analysis of Saponin Mixtures as Exemplified by the Deacylated and Partially Deacylated Triterpenoid Saponins of Bellis annua

Th. Schöpke1 , K. Hiller1 , V. Wray2 , M. Nimtz2
  • 1Institut für Pharmazie, Mathematisch-Naturwissenschaftliche Fakultät I, Humboldt-Universität zu Berlin, Goethestr. 54, D-13086 Berlin, Germany
  • 2Gesellschaft für Biotechnologische Forschung mbH, Mascheroder Weg 1, D-38124 Braunschweig, Germany
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Publication History

1995

1995

Publication Date:
04 January 2007 (online)

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Abstract

While most species of the Bellis genus are characterized by saponins in concentrations of ca.4% dry weight, Bellis annua only contains ca. 0.3 % saponins. Hence positive-ion electrospray ionization mass spectrometry, including MS-MS analysis, has been used extensively for the rapid identification of the saponins of B. annua obtained after mild alkaline hydrolysis. The saponin composition is similar to that of other Bellis species and, in addition, glycosides of the rare bellisonic acid, which has recently been found in B. bernardii, have been identified.

Key words

Bellis annua L. - Compositae - triterpenoid saponins - MS-MS - polygalacic acid - bellisonic acid

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