Planta Med 1996; 62(4): 293-296
DOI: 10.1055/s-2006-957887

© Georg Thieme Verlag Stuttgart · New York

Inhibition of Phospholipase Cγ1 Activity by Amentoflavone Isolated from Selaginella tamariscina

Hyun Sun Lee1 , Won Keun Oh1 , Bo Yeon Kim1 , Soon Cheol Ahn1 , Dae Ook Kang, Dong In Shin2 , Jinwoong Kim2 , Tae-lck Mheen1 , Jong Seog Ahn1
  • 1Korea Research Institute of Bioscience and Biotechnology (KRIBB), P.O. Box 115, Yusong, Taejon 305-600, Korea
  • 2College of Pharmacy, Seoul National University, Seoul 151-742, Korea
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Publication History



Publication Date:
04 January 2007 (online)


Amentoflavone was isolated as an inhibitor of phospholipase Cγ1 (PLCγ1) and phosphoinositides (Pl)-turnover in PLCγ1 overexpressing NIH3T3 fibroblasts (NIH3T3γ1) from Selaginella tamariscina (Selaginellaceae) together with other related biflavonoids, isocryptomerin and cryptomerin B. Only amentoflavone inhibited the PLCγ1 activity with an IC50 of 29 µM and the formation of total inositol phosphates (IPt) in PDGF-stimulated NIH3T3γ1 with an IC50 of 9.2 µM but did not show inhibitory activity against protein kinase C. Isocryptomerin and cryptomerin B did not show inhibitory activity against PLCγ1 at the concentration of 150 µM, and did not inhibit IPt production in PDGF-induced NIH3T3γ1 at the concentration of 180 µM.