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Planta Med 1998; 64(7): 645-648
DOI: 10.1055/s-2006-957539
Paper
Phytochemical Analysis
© Georg Thieme Verlag Stuttgart · New York

Quantitative Analysis of Anti-Emetic Principle in the Tubers of Pinellia ternata by Enzyme Immunoassay

Kiyoshi Kurata1 , Takaaki Tai1 , Ye Yang2 , Kaoru Kinoshita2 , Kiyotaka Koyama2 , Kunio Takahashi2 , Kazuo Watanabe3 , Yoshiki Nunoura1
  • 1Central Research Laboratory, Kotaro Pharmaceutical Co. Ltd., Osaka, Japan
  • 2Department of Pharmacognosy and Phytochemistry, Meiji College of Pharmacy, Tokyo, Japan
  • 3Faculty of Pharmaceutical Sciences, Chiba University, Chiba, Japan
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1997

1998

Publication Date:
04 January 2007 (online)

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Abstract

A crude globulin fraction was isolated from the tubers of Pinellia ternata (Thunb.) Breit. Tubers of P. ternata are one of the well known Chinese traditional medicines. It is used on account of its anti-emetic effect. 6KDP was separated by means of gel filtration and chromatography on CM-Toyopearl 650 M from the crude globulin fraction. 6KDP is a glycoprotein of M.W. 6kDa. 6KDP has an anti-emetic activity and hemagglutination activity. Both activities dropped during heat treatment. For the purpose to evaluate the quality of the powdered tubers of P. ternata, the quantitative analysis by means of EIA was established. The contents of 6KDP varied from 5.75 to 8.30%. The assay demonstrated a low cross-reaction with the crude globulin fraction of Colocasia antiquorum, a member of the same family as P. ternata. 6KDP is one of major proteins in the tubers of P. ternata. Thus 6KDP is a marker for quantitative analysis of the tubers of P. ternata.

Key words

Anti-emetic - quantitative analysis - enzyme immunoassay - Pinellia ternata (Thunb.) Breit. - Araceae