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© Georg Thieme Verlag Stuttgart · New York
Recombinant Expression of Alliin Lyase from Garlic (Allium sativum) in Bacteria and Yeasts
04 January 2007 (online)
Recombinant garlic alliin lyase was produced in Escherichia coli, Saccharomyces cerevisiae, and Pichia pastoris. A cDNA clone was obtained from garlic bulbs by PCR and introduced into suitable bacterial and yeast expression vectors. The recombinant alliin lyase forms inclusion bodies in all three host organisms, which are deposited in the cytoplasm. After cell lysis and harvesting by centrifugation, the inclusion bodies were solubilized in Zwittergent 3-14 solution and refolded by step-wise dilution. Specific alliin lyase activity could be recovered by this procedure.