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© Georg Thieme Verlag Stuttgart · New York
Phytochemical and Pharmacological Studies of Guettarda acreana
04 January 2007 (online)
The present study examines the effects of the extracts [petroleum ether, CHCl3, CHCl3/MeOH (9:1) and MeOH], partially purified fractions and pure compounds from Guettarda acreana on the electrically induced contractions (E.C.I.) of the isolated guinea-pig ileum. The results of the experiments indicate that CHCl3/MeOH (9:1), MeOH extract, and the MeOH soluble part from CHCl3/MeOH extract tested at concentrations of 1.2, 2.5, and 5 µg/ml, dose-dependently reduced the guinea-pig ileum contractions. Furthermore, some partially purified fractions I-IV from the MeOH extract, each tested at the same concentrations of the extracts, and some pure compounds (6 × 10-6, 3 × 10-6, 1 × 10-6 M) isolated from the above fractions significantly reduced, in a dose-dependent manner, the electrical contractions of the ileum. The active compounds were identified as the known indole alkaloids strictosidic acid, lyalosidic acid, 5α-carboxystrictosidine, strictosidine, and sickingine, as well as the known quinic acid derivatives 5-caffeoylquinic acid, 4,5-dicaffeoylquinic acid, and shikimic acid by spectral data. Two known quinovic acid glycosides and a new triterpenic glycoside, quinovic acid 3β-O-α-rhamnopyranosyl-(1→3)-(β-glucopyranosyl-(1→6)-β-glucopyranoside, were also isolated and their structures established by NMR and MS data.
Guettarda acreana - Rubiaceae - indole alkaloids - qunic acid derivatives - quinovic acid glycosides - electrical contractions - guinea-pig ileum - antispasmodic