Planta Med 1998; 64(3): 279-280
DOI: 10.1055/s-2006-957428
Letters
Letter
© Georg Thieme Verlag Stuttgart · New York

Rapid TLC-Densitometric Analysis of Plaunotol from Croton sublyratus Leaves

Aphacha Vongchareonsathit1 , 2 , Wanchai De-Eknamkul1
  • 1Department of Pharmacognosy, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok, Thailand
  • 2Present address: Department of Agro-Industry, Faculty of Agricultural Technology, King Mongkut's Institute of Technology Ladkrabang, Ladkrabang, Bangkok, Thailand
Further Information

Publication History

1997

1997

Publication Date:
04 January 2007 (online)

Abstract

A simple, rapid, and accurate method of TLC-densito-metry was developed to quantitate plaunotol, an antipeptic ulcer diterpenoid, in the leaves of Croton sublyratus Kurz (Euphor-biaceae). Plaunotol was extracted with boiling 95% ethanol and the unpurified extracts were used directly for the analysis of plaunotol by the TLC-densitometric method. TLC was carried out using silica gel and the solvent system of chloroform: n-propanol, 96:4. Densitometric scanning of the TLC plate was performed using the wavelength of 220 nm. The developed technique showed high accuracy and precision as compared with the GC method and could detect plaunotol at a quantity of as low as 0.12 µg. Preliminary experiments indicate that the technique can be a useful tool in the screening of Croton sublyratus plants containing high plaunotol content.