Planta Med 1998; 64(3): 259-263
DOI: 10.1055/s-2006-957421
Papers
Phytochemical Analysis
© Georg Thieme Verlag Stuttgart · New York

Generation and Characterization of Monoclonal Antibodies against the Pyrrolizidine Alkaloid Retrorsine

Ilse Zündorf1 , Helmut Wiedenfeld2 , Erhard Röder2 , Theodor Dingermann1
  • 1Institut für Pharmazeutische Biologie, Johann Wolfgang Goethe-Universität, Frankfurt, Germany
  • 2Pharmazeutisches Institut, Rheinische Friedrich-Wilhelms-Universität, Bonn-Endenich, Germany
Further Information

Publication History

1997

1997

Publication Date:
04 January 2007 (online)

Abstract

Monoclonal antibodies against retrorsine were established using a retrorsine-hemisuccinate::bovine-thyroglobuline conjugate as antigen for immunization. In competitive enzyme-linked immunoassays the specificity of the obtained antibodies was tested with 20 different pyrrolizidine alkaloids. Among these the alkaloids acetylgynuramine, gynuramine, integerrimine, neoplatyphylline, platyphylline, rosmarinine, senecionine, and seneciphylline were able to bind the antibody. There was no cross-reactivity with the alkaloids senkirkine, angeloyl-heliotridine, heliotridine, axillarine, anacrotine, monocrotaline, madurensine, otosenine, retronecine, tussilagine, triangularine, and jacoline as well as with seneciphylline N-oxide. From this antigen spectrum it can be concluded that besides the necic structure itself the dominant and discriminative epitope consists of the exocyclic ethylidene group of the various diesters.