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DOI: 10.1055/s-2006-957411
© Georg Thieme Verlag Stuttgart · New York
Anti-Tumor Effects of d-Dicentrine from the Root of Lindera megaphylla
Publication History
1997
1997
Publication Date:
04 January 2007 (online)
Abstract
d-Dicentrine, a naturally occurring aporphine type isoquinoline alkaloid, isolated from the root of Lindera megaphylla Hemsl. (Lauraceae), was evaluated for its potential anti-cancer activity. We found d-dicentrine significantly inhibited the growth of human hepatoma cell line HuH-7 by delaying its doubling time in tissue culture. An in vitro colony forming assay showed that d-dicentrine decreased the colony formation efficiency in both hepatoma cell lines, HuH-7 and MS-G2, used in our study. Biosyntheses of the macromolecules DNA and RNA were also strongly inhibited. An MTT assay in 21 tumor cell lines also revealed that d-dicentrine was most cytotoxic to esophageal carcinoma HCE-6, lymphoma cell lines Molt-4 and CESS, leukemia cell lines HL60 and K562, and hepatoma cell line MS-G2. An in vitro tumor growing assay in the Severe Combined Immunodeficiency (SCID) mice showed that intraperitoneal injection of d-dicentrine at the dose of 100 µg twice a week for 4 weeks significantly inhibited the tumor incidence of leukemia cell line K562 in SCID mice. All these data indicated that d-dicentrine has potential anti-tumor applications.
Key words
d-Dicentrine - Lindera megaphylla - Lauraceae - anti-tumor effect - MTT assay - SCID mice