Inhibition of Eukaryote Serine/Threonine-Specific Protein Kinases by Piceatannol

Abstract

The protein tyrosine kinase (PTK) inhibitor piceatannol is also an inhibitor of the rat liver cyclic AMP-dependent protein kinase (PKA) catalytic subunit (cAK), rat brain Ca²⁺- and phospholipid-dependent protein kinase C (PKC), avian gizzard Ca²⁺-calmodulin-dependent myosin light chain kinae (MLCK), and of wheat embryo Ca²⁺-dependent protein kinase (CDPK) (IC₅₀ values 3, 8,12, and 19 µM, respectively). However, a number of piceatannol-related compounds with fewer or no phenolic hydroxy substituents are inactive or very poor inhibitors of these serine/threonine protein kinases. Similarly, the PTK inhibitor ellagic acid is a potent inhibitor of cAK and of PKC (IC₅₀ values 2 and 8 µM, respectively), whereas the non-phenolic perylene is ineffective as a protein kinase inhibitor. Ellagic acid is a competitive inhibitor of both cAK and of PKC but piceatannol inhibits these enzymes in a fashion that is competitive and non-competitive, respectively. Interaction with calmodulin may contribute to the inhibition of MLCK and CDPK by piceatannol.

Key words

Protein kinase - piceatannol - stilbenes - ellagic acid