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Planta Med 1998; 64(1): 94-95
DOI: 10.1055/s-2006-957382
Letters

© Georg Thieme Verlag Stuttgart · New York

Reverse-Phase High Pressure Liquid Chromatographic Analysis of Harpagoside, Scorodioside and Verbascoside from Scrophularia scorodonia: Quantitative Determination of Harpagoside

Ana Díaz1 , Lidia Fernández1 , Evelyne Ollivier2 , Teresa Martín1 , Lucinda Villaescusa1 , G. Balansard2
  • 1Laboratory of Pharmacognosy, Faculty of Pharmacy, University of Alcalá de Henares Madrid, Spain
  • 2Laboratory of Pharmacognosy, Faculty of Pharmacy, Marseille, France
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1997

1997

Publikationsdatum:
04. Januar 2007 (online)

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Abstract

A reversed-phase high performance liquid chromatographic method has been developed for the determination of the main compounds (harpagoside, scorodioside, and verbascoside) from differents samples of Scrophularia scorodonia. The chromatographic method has been validated and applied for quantitative determination of harpagoside. The results show the highest levels of harpagoside in the leaf extract. The purity and identity of peaks were controlled by diode-array detection and comparison with standards.