Reverse-Phase High Pressure Liquid Chromatographic Analysis of Harpagoside, Scorodioside and Verbascoside from Scrophularia scorodonia: Quantitative Determination of Harpagoside

Ana Díaz; Lidia Fernández; Evelyne Ollivier; Teresa Martín; Lucinda Villaescusa; G. Balansard

doi:10.1055/s-2006-957382

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Planta Med 1998; 64(1): 94-95
DOI: 10.1055/s-2006-957382

Letters

Reverse-Phase High Pressure Liquid Chromatographic Analysis of Harpagoside, Scorodioside and Verbascoside from Scrophularia scorodonia: Quantitative Determination of Harpagoside

Ana Díaz¹ , Lidia Fernández¹ , Evelyne Ollivier² , Teresa Martín¹ , Lucinda Villaescusa¹ , G. Balansard²

¹Laboratory of Pharmacognosy, Faculty of Pharmacy, University of Alcalá de Henares Madrid, Spain
²Laboratory of Pharmacognosy, Faculty of Pharmacy, Marseille, France

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Publication History

1997

1997

Publication Date:
04 January 2007 (online)

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Abstract

A reversed-phase high performance liquid chromatographic method has been developed for the determination of the main compounds (harpagoside, scorodioside, and verbascoside) from differents samples of Scrophularia scorodonia. The chromatographic method has been validated and applied for quantitative determination of harpagoside. The results show the highest levels of harpagoside in the leaf extract. The purity and identity of peaks were controlled by diode-array detection and comparison with standards.