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DOI: 10.1055/s-2006-950072
Flavan-3-ols and procyanidins from the bark of Salix purpurea
From a hydroethanolic extract obtained from the bark of Salix purpurea L. (Salicaceae) the flavan-3-ols catechin, epicatechin, gallocatechin, catechin-3-O-(1-hydroxy-6-oxo-2-cyclohexene-1-carboxylic acid)-ester [1], the dimeric procyanidins B1, B3 [2] and the trimeric procyanidins epicatechin-(4ß8)-catechin-(48)-catechin [3] and epicatechin-(4ß8)-epicatechin-(4ß8)-catechin [4] were isolated. Their structures were elucidated by 1H-, 13C-NMR inlcuding COSY, HSCQ and HMBC methods and CD. The 13C-NMR spectral data of a fraction containing higher oligomeric procyanidins indicate an average degree of oligomerization of 4 to 5 flavan-3-ol units with dihydroxylated B-rings and predominance of the relative 2,3-cis-stereochemistry; B-ring trihydroxylated units were not detected.
Acknowledgements: Bionorica AG, Neumarkt (Germany), for financial support and the extract material.
References: 1. Hsu, F.L. et al. (1985), Phytochemistry 24: 2089–2092. 2. Kolodziej, H. (1986), Phytochemistry 25: 1209–1215. 3. Foo, L.Y., Karchesy, J.J. (1989), Phytochemistry 28: 1743–1747. 4. Shoji, T. et al. (2003), J. Agric. Food Chem. 51: 3806–3813.