Phytoestrogenic Activity of Morinda citrifolia L. Fruits

S. Basar; H. Iznaguen; A. Zeglin; J. Westendorf

doi:10.1055/s-2006-950034

Planta Medica, Table of Contents

Phytoestrogenic Activity of Morinda citrifolia L. Fruits

S Basar ¹, H Iznaguen ¹, A Zeglin ¹, J Westendorf ¹

¹Institute of Experimental and Clinical Pharmacology and Toxicology, University Clinic Hamburg Eppendorf, Vogt-Kölln-Straße 30, D-22527 Hamburg, Germany

Abstract

It is well known that certain plant metabolites can exert estrogenic activity. There are an increasing number of studies concerning the effects of phytoestrogen-rich diets which demonstrate that these plant metabolites have protective effects on estrogen-related conditions, such as menopausal symptoms, and estrogen-related diseases, such as prostate and breast cancers, osteoporosis and cardiovascular diseases [1].

Because beneficial effects on menopausal symptoms are often reported by women drinking regularly Noni fruit juice (Morinda citrifolia L.), we investigated the estrogenic capacity of Noni fruits in two in vitro assays, the estrogen receptor binding assay with both estrogen receptors, ER-α and ER-β, and the estrogen-receptor dependent induction of alkaline phosphatase in Ishikawa cells. Hexane extracts prepared from Noni fruit puree exhibited high activity in both systems. A preferential binding for ER-β was observed (ED₅₀(ER-α)/ED₅₀(ER-β)=2.33). Further analysis and fractionation of these extracts by HPLC showed that one compound is responsible for almost all the activity. The isolation of this constituent was performed by HPLC and will be followed by its characterisation with 1- and 2-dimensional NMR techniques and Mass spectrometry.

Reference: 1. Cos, P. et al. (2003), Planta Med. 69: 589–599.