Planta Med 2006; 72 - P_080
DOI: 10.1055/s-2006-949880

Effects of constituents from the fruits of Idesia polycarpa on lipopolysaccharide-induced nitric oxide production in BV2 microglial cells

SH Kim 1, EJ Jeong 1, NR Ha 1, H Yang 1, S Hyun 1, S Young 1, C Kim 1
  • 1College of Pharmacy, Seoul National University (a), San 56–1, Shillim-Dong, Kwanak-Gu, 151–742, Seoul, Korea

In our search for anti-inflammatory substances from natural products, we found that the total methanol extract of the fruits of Idesia polycarpa effectively inhibited nitric oxide (NO) production induced by lipopolysaccharide (LPS) in BV2 microglial cells [1]. Through the bioactivity-guided fractionation of this extract, four compounds (1˜4), idescorpin (1), idesin (2), 1-hydroxy-6-oxocyclohex-2-enoic acid methyl ester (3) and salirepin (4), were isolated from the ethylacetate fraction. These compounds significantly inhibited LPS-induced NO production in BV2 microglial cells despite of their weak NO radical scavenging activity. Moreover, they showed significant inhibitory effect on inducible NO synthase (iNOS) activity without affecting iNOS expression as demonstrated by Western blot analysis.

Acknowledgements: Brain Research Center of the 21st Century Frontier Research Program funded by the Ministry of Science and Technology, the Republic of Korea

Reference: 1. Kim, S.H. et al. (2005), Org. Lett. 7: 3275–3277.