A New Triterpene and Anti-Hepatitis B Virus Active Compounds from Alisma orientalis

Abstract

A new triterpenoid named alisol O (1) was isolated from the rhizomes of Alisma orientalis, together with six known compounds: alisol A 24-acetate (2), 25-anhydroalisol A (3), 13β,17β-epoxyalisol A (4), alisol B 23-acetate (5), alisol F (6), and alisol F 24-acetate (7). Based on 1D and 2D-NMR data (HMQC, HMBC, COSY, ROESY), the structure of the new compound was deduced to be 11-dehydroxy-12-dehydroalisol F-24-acetate (1). Compounds 2 - 7 exhibited inhibitory activity in vitro on hepatitis B virus (HBV) surface antigen (HBsAg) secretion of the Hep G2.2.15 cell line with IC₅₀ values of 2.3, 11.0, 15.4, 14.3, 0.6 and 7.7 μM, and on HBV e antigen (HBeAg) secretion with IC₅₀ values of 498.1, 17.6, 41.0, 19.9, 8.5 and 5.1 μM, respectively.

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