Neuropediatrics 2006; 37 - THP38
DOI: 10.1055/s-2006-945861

ACTION OF BRAIN-DERIVED NEUROTROPHIC FACTOR ON BRAIN DAMAGE INDUCED BY STATUS CONVULSION

L Jiang 1, Y Hu 1
  • 1Children's Hospital, Chongqing University of Medical Sciences, Chongqing, P.R. China

Objectives: To investigate whether the exogenous BDNF or the phosphorylation of cAMP response element-binding protein (CREB) influence the hippocampal BDNF expression and the apoptotic procedure in vivo.

Methods: Seizures were induced in Wistar rats with lithium and pilocarpine injected intraperitoneally. The rats were intraventricular (IV) injected with BDNF or antipCREB antibody after SC, and sacrificed 1d later. The level of BDNF in hippocampus of both side were determined by quantitative ELISA. The proportion of apoptotic cells was quantified by the Annexin V-FITC apoptosis detection. The expression of pCREB in hippocampus after 30min of SC were observed by semiquantitative immunohistochemistry.

Results: (1) The level of BDNF in the hippocampus of the lateral received IV injection of BDNF were significantly increased in the normal group and SC group, and the level was much higher in SC group (55.40±4.11 pg /ìg) compared with the normal group (p 0.01). However, the proportion of apoptotic cells in the same aera was remarkably decreased (p 0.01). (2) Expression of pCREB in the granule cells were significantly increased at 3h following 30min of SC. In the lateral received IV of antipCREB antibody in the normal group, BDNF levels were not significantly increased compared with the blank control;but the level of BDNF in the SC group (5.53±1.11 pg /ìg) were dropped dramatically (p 0.01). and the proportion of apoptotic cells was remarkably increased, compared with the normal groups and the SC group (IV injection of NS). (3) The intraventricular injection could induce neuronal apoptosis in the lateral injected, but had no significant influence on the contralateral.

Conclusion: (1) The exogenous BDNF could induced the expression of endogenous BDNF and partly inhibit neuronal apoptosis in the lateral hippocampus of intraventricular injection. (2) The blockade of CREB phosphorylation induced the apoptotic procedure, probably through suppressing the BDNF expression.