A Model of Unilateral Frontal Focal Cerebral Venous Occlusion in the Rat
18 July 2006 (online)
Objective: The aim of this experimental study was to create a new focal venous infarction model in rats. Method: 20-male Sprague-Dawley rats were used in this experiment. Unilateral frontal strip craniectomy was done in front of the coronal suture. Frontal veins were observed by using the operative microscope. In this study we used the bipolar coagulation method over the transparent dura mater for occlusion of the bridging veins for venous infarction. Specimens were evaluated by histopathological techniques. Results: Unilateral frontal hemispheric swelling, midline shift, brain edema, subcortical petechial hemorrhage, hemorrhagic infarction and necrosis were the histopathological findings on microscopic examination. Conclusion: Our results revealed that the bipolar coagulation method over the transparent dura mater for occlusion of the unilateral frontal cortical veins for venous infarction might be a new experimental model in the evaluation of brain damage after disturbance to the venous circulation.
Unilateral cortical veins - brain swelling - focal venous infarction model - rat brain
- 1 Kawaguchi T, Kawano T, Kaneko Y, Ooasa T, Masanori T, Ogasawara S. Classification of venous ischemia with MRI. J Clin Neurosci. 2001; 8 (Supplement 1) 82-88
- 2 Nakase H, Kakizaki T, Kazunori M, Hiramatsu K, Sakaki T. Use of local cerebral blood flow monitoring to predict brain damage after disturbance to the venous circulation: cortical vein occlusion model by photochemical dye. Experimental study. Neurosurgery. 1995; 37 280-286
- 3 Benamer H TS. Cerebral venous thrombosis: anticoagulants or thrombolyic therapy?. J Neurol Neurosurg Psychiatry. 2000; 69 427-430
- 4 Fries G, Wallenfang T, Hennen J, Velthaus M, Heimann A, Schild H, Perneczky A, Kempski O. Occlusion of the pig superior sagittal sinus, bridging and cortical veins: multistep evolution of sinus-vein thrombosis. J Neurosurg. 1992; 77 127-133
- 5 Nakase H, Kempski O S, Heimann A, Takeshima T, Tintera J. Microcirculation after cerebral venous occlusion as assessed by laser Doppler scanning. J Neurosurg. 1997; 87 307-314
- 6 Forbes K PN, Pipe J G, Heiserman J E. Evidence for cytotoxic edema in the pathogenesis of cerebral venous infarction. AJNR Am J Neuroradiol. 2001; 22 450-455
- 7 Schaller B, Graf R, Weinbard K, Heiss W D. A new animal model of cerebral venous infarction: ligation of the posterior part of the superior sagittal sinus in the cat. Swiss Med Wkly. 2003; 133 412-418
- 8 Garcia J H, Liu K F, Yoshida Y, Lian J, Chen S, Del Zoppo G J. Influx of leukocytes and platelets in an evolving brain infarct (Wistar rat). Am J Pathol. 1994; 144 188-199
- 9 Narayanan U, Weiss H R, Liu X, Chi O Z. Exogenous endothelin-1 improves microvascular oxygen balance during focal cerebral ischemia in the rat. Regul Pept. 2002; 105 1-7
- 10 Gomi S, Burnett M G, Karp A, Greenberg J H. Nimodipine does not affect the flow-metabolism couple in permanent cerebral ischemia. Exp Brain Res. 2004; 155 469-476
- 11 Krayenbuhl H, Yasargil M G. Cerebral angiography, 2nd edn. Stuttgart: Georg Thieme Verlag 1982: 181-235
- 12 Yasargil M G. Microneurosurgery IVA. Stuttgart: Georg Thieme Verlag 1994: 109-114
- 13 Di Chiro G. Angiographic patterns of cerebral convexity veins and superficial dural sinuses. Am J Roentgenol. 1962; 87 308-321
- 14 Delmas A, Chifflet J. Le sinus longitudinal superieur et les voies de drainage de la convexite cerebrale. Sem Hop Paris. 1950; 26 4888
Keramettin Aydın,, M. D.
Department of Neurosurgery · Medical Faculty · Ondokuzmayıs University
Phone: +90/362/312/1919/3652 int.
Fax: +90/362/457/6041 ·