Deoxypodophyllotoxin (DPT) Inhibits Eosinophil Recruitment into the Airway and Th2 Cytokine Expression in an OVA-Induced Lung Inflammation

Abstract

The effect of deoxypodophyllotoxin (DPT) isolated from Anthriscus sylvestris Hoffm. was evaluated in an in vivo animal model for antiasthmatic activity. DPT (1.0 to 5 mg/kg) was given orally to ovalbumin (OVA)/alum-induced asthmatic mice. DPT reduced the number of infiltrated eosinophils in bronchoalveolar lavage (BAL) fluid in a dose-dependent manner. Dexamethasone (5 mg/kg), which was used as a positive control, also strongly inhibited the number of infiltrated eosinophils. The effect of DPT on a transcript profile in a murine asthma model was determined by RT-PCR, which showed that DPT decreased the mRNA levels of the Th2 cytokines. Northern blot analysis showed that DPT also reduced both the eotaxin and arginase I mRNA levels in a dose-dependent manner.

Key words

Deoxypodophyllotoxin (DPT) - eosinophils - bronchoalveolar lavage (BAL) - asthma - Th2 cytokines

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