Abstract
Ginseng has been used as a powder or a crude extract of the plant roots. The quality control of commercial ginseng preparations is difficult due to the diverse compounds present. Most previous quality control methods using TLC or HPLC-UV (or -MS) cannot be expected to cover a wide range of compounds in the commercial ginseng preparations. In this study, the metabolic fingerprinting of ginseng preparations was performed by 1H-NMR spectroscopy. Although 1H -NMR spectroscopy could provide information about the total profile of the compounds present, low resolution and overlapping signals make it difficult for further identification of each compound. For overcoming the problem two-dimensional J-resolved NMR spectra and multivariate data analysis techniques was applied for the analysis. Principal component analysis (PCA) of projected J-resolved NMR spectra shows a clear discrimination among those samples by principal component 1 and principal component 3. The loading plot of PC values obtained from all NMR signals indicates that alanine, arginine, choline, fumaric acid, inositol, sucrose as well as ginsenosides are important metabolites to differentiate the preparations from each other. This method allows an efficient discrimination of a ginseng preparation in less than 15 minutes without any pre-purification steps.
Key words
Ginseng - metabolic fingerprinting - NMR -
J-resolved spectrum - principal component analysis
References
-
1
Grandhi A, Mujumdar A M, Patwardhan B A.
Comparative pharmacological investigation of Ashwagandha and Ginseng.
J Ethnopharmacol.
1994;
44
131-5
-
2 Soldati F.
Panax ginseng: standardization and biological activity. In: Biologically active natural products: pharmaceuticals. Cutler SJ, Cutler HG, editors New York; CRC Press 2000: pp 209-32
-
3
Corthout J, Nassessens S, Apers A J, Vlietinck A J.
Quantitative determination of ginsenosides from Panax ginseng roots and ginseng preparations by thin layer chromatography-densitometry.
J Pharm Biomed Anal.
1999;
21
187-92
-
4
Chuang W C, Sheu S J.
Determination of ginsenosides in ginseng crude extracts by high performance liquid chromatography.
J Chromatogr A.
1994;
685
243-51
-
5
Kwon S W, Han S B, Park H I, Kim J M, Park M K, Park J H.
Liquid chromatographic determination of less polar ginsenosides in process ginseng.
J Chromatogr A.
2001;
921
335-9
-
6
Bonfill M, Casals I, Palazon J, Mallol A, Morales C.
Improved high performance liquid chromatographic determination of ginsenosides in Panax ginseng-based pharmaceuticals using a diol column.
Biomed Chromatogr.
2002;
16
68-72
-
7
Washida D, Kitanaka S.
Determination of polyacetylenes and ginsenosides in Panax species using high performance liquid chromatography.
Chem Pharm Bull.
2003;
51
1314-7
-
8
Dou D Q, Ren J, Chen Y, Pei Y P, Chen Y J.
Study on the chemical constituents of the roots of commercial ginseng.
Med J China.
2003;
28
522-4
-
9
Cai Z W, Lee F SC, Wang X R, Yu W J.
A capsule review of recent studies on the application of mass spectrometry in the analysis of Chinese medicinal herbs.
J Mass Spectrom.
2002;
37
1013-24
-
10
Popovich D, Kitts D D.
Generation of ginsenosides Rg3 and Rh2 from North American ginseng.
Phytochemistry.
2004;
65
337-44
-
11
Nam M H, Heo E J, Kim J Y, Kim S, Kwon K H, Seo J B. et al .
Proteome analysis of the responses of Panax ginseng C. A. Meyer leaves to high light: use of electrospray ionization quadrupole-time of flight mass spectrometry and expressed sequence tag data.
Proteomics.
2003;
3
2351-67
-
12
Liu S Y, Cui M, Liu Z Q, Song F R, Mo W J.
Structural analysis of saponins from medicine herbs using electrospray ionization tandem mass spectrometry.
J Am Soc Mass Spectrom.
2004;
15
33-41
-
13
Cui J F.
Identification and quantification of ginsenosides in various commercial ginseng preparations.
Eur J Pharm Sci.
1995;
3
77-85
-
14
Hou D, Zhang W, Hui R.
Separation and determination of chemical constituents in the volatile oil of three traditional Chinese crude drugs.
J Pharm Biomed Anal.
1998;
17
1423-6
-
15
Wang X M, Takeo S, Ebenezer A, Gerals K S.
Determination of ginsenosides in plant extracts from Panax ginseng and Panax quinquefolius L. by LC/MS/MS.
Anal Chem.
1999;
71
1579-84
-
16
Cui M, Song F, Zhou Y, Liu Z, Liu S.
Rapid identification of saponins in plant extracts by electrospray ionization multi-stage tandem mass spectrometry and liquid chromatography/tandem mass spectrometry.
Rapid Commun Mass Spectrom.
2000;
14
1280-6
-
17
Harkey M R, Henderson G L, Gershwin M E, Stern J S, Hackman R M.
Variability in commercial ginseng products: an analysis of 25 preparations.
Am J Clin Nutr.
2001;
73
1101-6
-
18
Sumner L W, Mendes P, Dixon R A.
Plant metabolomics: large-scale phytochemistry in the functional genomics era.
Phytochemistry.
2003;
62
718-36
-
19
Choi Y H, Kim H K, Hazekamp A, Erkelens C, Lefeber A WM, Verpoorte R.
Metabolomic differentiation of Cannabis sativa cultivars using 1H NMR spectroscopy and principal component analysis.
J Nat Prod.
2004;
67
953-7
-
20
Frederich M, Choi Y H, Angenot L, Harnischfeger G, Lefeber A WM, Verpoorte R.
Metabolomic analysis of Strychnos nux-vomica, Strychnos icaja and Strychnos ignatii extracts by 1H nuclear magnetic resonance spectrometry and multivariate analysis techniques.
Phytochemisty.
2004;
65
1993-2001
-
21
Choi Y H, Sertic S, Kim H K, Wilson E G, Michopoulosa F, Lefeber A WM. et al .
Classification of Ilex species based on metabolomic fingerprinting using NMR and multivariate data analysis.
J Agric Food Chem.
2005;
53
1237-45
-
22
Viant M R.
Improved method for the acquisition and interpretation of NMR metabolomic data.
Biochem Biophys Res Commun.
2003;
310
943-8
-
23 Shoji J. Recent advances in the chemical studies on ginseng. In: Advances in chinese medicinal materials research. Chang HM, Yeung HW, Tso WW, Koo A, editors Singapore; World Scientific 1985: pp 455-69
-
24
Tomada M, Shimada K, Konno C, Sugiyama K, Hikino H.
Validity of oriental medicines, LXXI. Antidiabetes drugs. 2. Partial structure of panaxan A, a hypoglycemic glycan of Panax ginseng roots.
Planta Med.
1984;
50
436-8
-
25
Tomada M, Shimada K, Konno C, Hikino H.
Validity of oriental medicines, XCIV. Antidiabetes drugs. 12. Structure of panaxan B, a hypoglycemic glycan of Panax ginseng roots.
Phytochemistry.
1985;
24
2431-3
Young Hae Choi
Division of Pharmacognosy
Section Metabolomics
Institute of Biology
Leiden University
P.O. Box 9502
2300 RA Leiden
The Netherlands
Telefon: +31-71-527-4471
Fax: +31-71-527-4511
eMail: y.choi@chem.leidenuniv.nl
