Abstract
Airway inflammation and remodeling in chronic asthma are characterized by airway eosinophilia,
hyperplasia of smooth muscle and goblet cells, and subepithelial fibrosis. The present
study was undertaken to evaluate the effects of DA-9201, an ethanolic extract of black
rice (Oryza sativa L.), on airway inflammation and remodeling in a murine model of chronic asthma. BALB/c
mice sensitized to ovalbumin (OVA) were chronically challenged with aerosolized OVA
for 6 weeks. DA-9201 (30, 100, or 300 mg/kg) or dexamethasone (3 mg/kg) was orally
administered during the last 4 and 2 weeks, respectively. Airway inflammation, lung
pathology by histomorphometry and immunohistochemistry, IgE level and Th2 cytokines
were evaluated. The OVA-treated mice showed extensive eosinophilia, chronic inflammatory
responses and characteristics of airway remodeling including subepithelial fibrosis,
smooth muscle hypertrophy, and goblet cell hyperplasia. As compared to the OVA-treated
control group, treatment with DA-9201 resulted in significant reductions in the accumulation
of eosinophils in peribronchial areas, chronic pulmonary inflammation and progression
of airway remodeling. Furthermore, DA-9201 significantly reduced total serum and BALF
IgE levels and Th2 cytokines. These results indicate that DA-9201 may play an important
role in attenuating the progressing of airway inflammation and remodeling and suggest
the potential benefits of DA-9201 in prevention or treatment of asthma.
Abbreviations
OVA:ovalbumin
BALF:bronchoalveolar lavage fluid
PAS:periodic acid - Schiff
α-SMA:α-smooth muscle actin
BSA:bovine serum albumin
PBS:phosphate buffered saline
EDTA:ethylenediaminetetraacetic acid
H & E:hematoxylin and eosin
ELISA:enzyme linked immunosorbent assay
IL:interleukin
Penh:enhanced pause
IgE:immunoglobulin E
MT:masson's trichrome
Key words
Chronic asthma - pulmonary inflammation - airway remodeling -
Oryza sativa
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Kyung Koo Kang
Research Institutes of Dong-A Pharmaceutical Company
47-5, Sanggal
Kiheung
Yongin
Kyunggi 449-905
Republic of Korea
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