Time-Course Accumulation of Main Bioactive Components in the Rhizome of Ligusticum chuanxiong

Abstract

Fresh rhizomes of Ligusticum chuanxiong, a commonly used traditional Chinese medicinal herb, were collected monthly from a cultivating base in China practicing good agriculture practice (GAP). These samples were analyzed by HPLC-UV for their main chemical ingredients. Senkyunolide A (6), coniferyl ferulate (7) and Z-ligustilide (8) were identified as the major ingredients. The accumulation of the main ingredients with time in the herb was elucidated. Both individual and total contents of all main components gradually increased from the beginning of October to the middle of next April. The weight of a single rhizome reached a plateau at the end of May, whilst the content of the major ingredients peaked in the middle of April. Based on these results, it is recommended that Rhizoma Chuanxiong be harvested between the middle of April and the end of May.

References

• 1 Hu S L, Chi Q, Zhao Z Z. The authentic and superior medicinal herbals in China. Haerbin; Heilongjiang Press of Science and Technology 1989: p. 133-7
• 2 Wang P S, Gao Y X, Wang Y X, Yoshiyasu F, Iwao M, Michiharu S. Phthalides from the rhizome of Ligusticum wallichi .  Phytochemistry. 1984;  23 2033-8
• 3 Wang P S. Survey of investigation on chemical principles and biological activities of Ligusticum wallichi .  Zhongguo Yiyao Gongye. 1988;  19 553-9
• 4 Kobayashi M, Fujita M, Mitsuhashi H. Studies on the constituents of Umbelliferae plants. XV. Constituents of Cnidium officinale: Occurrence of pregnenolone, coniferyl ferulate and hydroxyphthalides.  Chem Pharm Bull. 1987;  35 1427-33
• 5 Tsuchida T, Kobayashi M, Kaneko K, Mitsuhashi H. Studies on the constituents of Umbelliferae plants. XVI. Isolation and structures of three new ligustilide derivatives from Angelica acutiloba .  Chem Pharm Bull. 1987;  35 4460-4
• 6 Kobayashi M, Mitsuhashi H. Studies on the constituents of Umbelliferae plants. XVII. Structures of three new ligustilide derivatives from Ligusticum wallichi .  Chem Pharm Bull. 1987;  35 4789-92
• 7 Kaouadji M, Pachtere F, Pouget C, Chulia A J. Three additional phthalide derivatives, an epoxymonomer and two dimers, from Ligusticum wallichi rhizomes.  J Nat Prod. 1986;  49 872-7
• 8 Naito T, Katsuhara T, Niitsu K, Ikeya Y, Okada M, Mitsuhashi H. Phthalide dimers from Ligusticum chuanxiong Hort.  Heterocycles. 1991;  32 2433-42
• 9 Naito T, Kubota K, Shimoda Y, Sato T, Ikeya Y, Okada M. Effects of constituents in a Chinese crude drug, Ligustici Chuanxiong Rhizoma on vasocontracton and blood viscosity.  Nat Med. 1995;  49 288-92
• 10 Kobayashi S, Mimura Y, Notoya K, Kimura I, Kimura M. Antiproliferative effects of the traditional Chinese medicine Shimotsu-To, its component Cnidium rhizome and derived compounds on primary cultures of mouse aorta smooth muscle cells.  Jap J Pharmacol. 1992;  60 397-401
• 11 Kobayashi S, Mimura Y, Naito Y, Kimura I, Kimura M. Chemical structure-activity of Cnidium rhizome-derived phthalides for the competence inhibition of proliferation in primary cultures of mouse aorta smooth muscle cells.  Jap J Pharmacol. 1993;  63 353-9
• 12 Foye W O, Wang H P, Wang H F. Synthesis and platelet aggregation inhibitory effects of harman and phthalide derivatives related to Ligusticum chuanxiong Hort. constituents.  Med Chem Res. 1997;  7 180-91
• 13 Li S L, Chan S SK, Lin G, Ling L, Yan R, Chung H S. et al . Simultaneous analysis of seventeen chemical ingredients of Ligusticum chuanxiong by on-line high performance liquid chromatography-diode array detector-mass spectrometry.  Planta Med. 2003;  69 445-51
• 14 Yan R, Li S L, Chung H S, Tam Y K, Lin G. Simultaneous quantification of 12 bioactive components of Ligusticum chuanxiong Hort. by high-performance liquid chromatography.  J Pharm Biomed Anal. 2005;  37 87-95
• 15 Li S L, Lin G, Chung H S, Tam Y K. Study on fingerprint of Rhizoma Chuanxiong by HPLC-DAD-MS.  Acta Pharm Sin. 2004;  39 621-6
• 16 Peking Institute of Pharmaceutical I ndustries. Structure elucidation and synthesis of tetramethylpyrazine.  Zhong Cao Yao. 1977;  8 150-2
• 17 Chen X F, Ding D R, Liu S R, Huang W X, Liu S X. Studies on biological characters of Ligusticum chuanxiong Hort.  Zhongguo Zhongyao Zazhi. 1994;  19 463-6
• 18 Chen X F, Ding D R, Huang W X, Liu S R, Liu S X. Investigations into biological characters of Ligusticum chuanxiong Hort. during growth.  Zhongguo Zhongyao Zazhi. 1997;  22 527-9