Planta Med 2005; 71(12): 1152-1156
DOI: 10.1055/s-2005-873151
Original Paper
Analytical Methods
© Georg Thieme Verlag KG Stuttgart · New York

Determination of Five Coumarins in Cnidii Fructus by Micellar Electrokinetic Capillary Chromatography

Meng Hua Wu1 , Lu Hua Zhao1 , Yue Song1 , Wei Zhang2 , Bing Ren Xiang1 , Ling Hua Mei3
  • 1Key Laboratory of Modern Chinese Medicine, Ministry of Education, Nanjing, P.R. China
  • 2Guangzhou Institute for Drug Control, Guangzhou, P.R. China
  • 3Jiangxi Huiren Pharmaceutical Co., Ltd., Nanchang, P.R. China
Further Information

Publication History

Received: March 26, 2005

Accepted: May 30, 2005

Publication Date:
10 November 2005 (online)

Abstract

A micellar electrokinetic capillary chromatography (MEKC) method for the separation and quantification of the coumarins in Cnidii Fructus was developed for the first time. Within 25 minutes, 5 major coumarins were separated using 18 mM borate, 12 mM phosphate and 50 mM SDS (pH 9.2) containing 20 % methanol. The relative standard deviations of migration times and peak areas were less than 5 %. The total contents of the five coumarins in plants from different habitats ranged from 13.6 to 44.6 mg/g, and recoveries ranged from 96.0 to 102.8 %. The effects of buffer concentration, SDS, pH value and organic modifier on the separation were investigated.

References

Lu Hua Zhao

Key Laboratory of Modern Chinese Medicine

Ministry of Education

Nanjing

People’s Republic of China

Phone: +86-25-8327-1185

Fax: +86-25-8327-1399

Email: zhaoluhua@hotmail.com