Planta Med 2005; 71(12): 1167-1170
DOI: 10.1055/s-2005-873147
Letter
© Georg Thieme Verlag KG Stuttgart · New York

Anti-Inflammatory Activity of 20(S)-Protopanaxadiol: Enhanced Heme Oxygenase 1 Expression in RAW 264.7 Cells

Sung Hee Lee1 , Geom Seog Seo2 , Geonil Ko1 , Jae Baek Kim3 , Dong Hwan Sohn1
  • 1College of Pharmacy, Medicinal Resources Research Institute, Wonkwang University, Iksan, Jeonbuk, Republic of Korea
  • 2Department of Internal Medicine, Wonkwang University Medical School, Iksan, Jeonbuk, Republic of Korea
  • 3Wonkwang Pharmaceutical Co. Ltd., Iksan, Jeonbuk, Republic of Korea
Further Information

Publication History

Received: December 19, 2004

Accepted: June 27, 2005

Publication Date:
23 November 2005 (online)

Abstract

20(S)-Protopanaxadiol (PPD) is one of the metabolites of ginsenosides from Panax ginseng. In this study, we demonstrate that PPD inhibits the increase in lipopolysaccharide (LPS)-induced inducible nitric oxide synthase (iNOS) expression through inactivation of nuclear factor-κB by preventing degradation of inhibitory factor-κBα. PPD also induces heme oxygenase 1 (HO-1) expression in RAW 264.7 cells, at the mRNA and protein levels, in the presence and absence of LPS. This effect is associated with suppression of LPS-induced nitric oxide (NO) production and iNOS expression. The HO-1 inducer hemin is associated with the suppression of LPS-induced NO production in a dose-dependent manner, and the HO-1 inhibitor tin protoporphyrin attenuates the inhibitory activity of PPD on LPS-induced NO production. These results provide evidence for the role of HO-1 in the inhibition of LPS-induced NO production by PPD.

References

Prof. Dong Hwan Sohn

College of Pharmacy

Medicinal Resources Research Institute

Wonkwang University

Iksan

Jeonbuk 570-749

Republic of Korea

Phone: +82-63-850-6822

Fax: +82-63-854-6038

Email: dhsohn@wonkwang.ac.kr