Planta Med 2005; 71(12): 1134-1139
DOI: 10.1055/s-2005-873138
Original Paper
Physiology and in vitro Biotechnology
© Georg Thieme Verlag KG Stuttgart · New York

The Effect of Genotype and Cultivation Method on the Total Salicylate Yield of Dark-Leaved Willows (Salix myrsinifolia)

S. Heiska1 , M. Rousi2 , S. Turtola1 , B. Meier3 , V. Tirkkonen4 , R. Julkunen-Tiitto1
  • 1Natural Product Research Laboratory, University of Joensuu, Department of Biology, Joensuu, Finland
  • 2Finnish Forest Research Institute, Punkaharju Research Station, Punkaharju, Finland
  • 3University of Applied Sciences, Wädenswil, Switzerland
  • 4Tapvei Ltd., Kortteinen, Finland
Further Information

Publication History

Received: February 25, 2005

Accepted: May 30, 2005

Publication Date:
20 October 2005 (online)


Ten clones of dark-leaved willows (Salix myrsinifolia Salisb.) were grown for two years on two different sites, Luikonlahti and Punkaharju, using various combinations of soil cultivation, fertilization and mulch treatments, with the objective of comparing the effect of different cultivation methods on the growth and total salicylate yield. In cultivated soil, the total salicylate yield ranged from 20 to 220 kg/ha, depending on the clone and cultivation method. The use of black polythene mulch and the addition of mineral fertilizer reduced the total salicylate concentrations of several clones. On the other hand, polythene mulch noticeably increased the hectare yield of total salicylates by promoting biomass accumulation.


LE:Luikonlahti experiment

PE:Punkaharju experiment


S. Heiska

Natural Product Research Laboratory

Department of Biology

University of Joensuu

P.O. Box 111

FI-80101 Joensuu