Planta Med 2005; 71(12): 1099-1105
DOI: 10.1055/s-2005-873137
Original Paper
Pharmacology
© Georg Thieme Verlag KG Stuttgart · New York

Antihyperglycemic Effect of Constituents from Hintonia standleyana in Streptozotocin-Induced Diabetic Rats

José A. Guerrero-Analco1 , 3 , Paul Hersch-Martínez2 , José Pedraza-Chaverri1 , Andrés Navarrete1 , Rachel Mata1
  • 1Facultad de Química, Universidad Nacional Autónoma de México, Ciudad Universitaria, México DF, México
  • 2Instituto Nacional de Antropología e Historia, Cuernavaca, Morelos, México
  • 3Taken in part from the PhD thesis of J. A. Guerrero-Analco submitted to Posgrado en Ciencias Químicas, UNAM, 2005
Further Information

Publication History

Received: January 19, 2005

Accepted: May 27, 2005

Publication Date:
10 November 2005 (online)

Abstract

An extract (100 mg/kg) of the stem bark of Hintonia standleyana caused a significant decrease in blood glucose levels in both normal and streptozotocin (STZ)-diabetic rats when compared with vehicle-treated groups (p < 0.05). From the active extract, 3-O-β-D-glucopyranosyl-23,24-dihydrocucurbitacin F (1), 5-O-β-D-glucopyranosyl-7-methoxy-3′,4′-dihydroxy-4-phenylcoumarin (2) and 5-O-[β-D-apiofuranosyl-(1→6)-β-D-glucopyranosyl]-7-methoxy-3′,4′-dihydroxy-4-phenylcoumarin (3) were isolated. Coumarin 3 is a new natural product and was identified by spectroscopic methods. Compounds 1 and 3 did not decrease blood glucose levels in normal rats. However, in two different long-term subacute experiments, using animals with a developing diabetes condition and with STZ-induced diabetes, both compounds at daily doses of 10 mg/kg (developing diabetes condition) or 30 mg/kg (STZ-induced diabetes condition) provoked a significant antihyperglycemic activity (p < 0.05). Furthermore, compound 3 restored normal blood glucose levels in STZ-induced diabetic rats. In all cases, the groups treated with the active principles and the extract showed less body weight lost than the glibenclamide-treated and diabetic control groups (p < 0.05). These results showed that the antihyperglycemic active principles of H. standleyana are both 4-phenylcoumarins and cucurbitacin glycosides.

References

Dr. Rachel Mata

Facultad de Química

Universidad Nacional Autónoma de México

Coyoacán 04510

México City

México

Phone: +52-5-5622-5289

Fax: +52-5-5622-5329

Email: rachel@servidor.unam.mx