Möglichkeiten und Grenzen der Mikrogefäßdarstellung

Zusammenfassung

Für Untersuchungen des Gefäßsystems und der terminalen Strombahn steht eine Reihe komplementärer morphologischer Untersuchungsverfahren zur Verfügung, die zu einer verbesserten Struktur-Funktions-Analyse eingesetzt werden. Zweidimensionale licht- und elektronenoptische Schnittverfahren mit oder ohne spezifische Markierungen werden durch dreidimensionale Visualisierungsmöglichkeiten der Vaskularisation und der Perfusion ergänzt. Noch dominieren die statischen, Ex-vivo-Untersuchungsmethoden, die Zukunft wird jedoch von intravitalen Methoden bestimmt werden.

Abstract

Vessel systems and the microvascular unit may be studied by a variety of morphological techniques which enable improved structure-function analyses. Two-dimensional light and electron microscopic sectioning techniques with and without specific markers are complemented by 3D vascularisation and perfusion study techniques. Static ex vivo modalities still prevail, however, the future will be dominated by intravital high-resolution techniques.

Schlüsselwörter

Angiogenese - Morphologie - Immunohistochemie - Gefäßdichte - konfokale Mikroskopie - Corrosion Casting - Elektronenmikroskopie

Key words

Angiogenesis - morphology - immunostaining - microvessel density count - confocal microscopy - corrosion casting - electron microscopy

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