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Plant Biol (Stuttg) 2005; 7(5): 459-468
DOI: 10.1055/s-2005-865851
Research Paper

Georg Thieme Verlag Stuttgart KG · New York

Up-Regulation of OsBIHD1, a Rice Gene Encoding BELL Homeodomain Transcriptional Factor, in Disease Resistance Responses

H. Luo1 , 2 , 5 , F. Song1 , 2 , 3 , R. M. Goodman4 , Z. Zheng1 , 2
  • 1Institute of Biotechnology, Zhejiang University, Hangzhou, Zhejiang 310029, P.R. China
  • 2Department of Plant Protection, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, Zhejiang 310029, P.R. China
  • 3State Key Laboratory of Rice Biology, Zhejiang University, Hangzhou, Zhejiang 310029, P.R. China
  • 4Department of Botany and Plant Pathology, Gaylord Nelson Institute for Environmental Studies, University of Wisconsin, Madison, WI 53706, USA
  • 5Present address: Institute of Biotechnology, Hainan University, Haikou, Hainan 570000, P.R. China
Further Information

Publication History

Received: March 23, 2005

Accepted: May 30, 2005

Publication Date:
15 September 2005 (online)

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Abstract

In the present study, we cloned and identified a full-length cDNA of a rice gene, OsBIHD1, encoding a homeodomain type transcriptional factor. OsBIHD1 is predicted to encode a 642 amino acid protein and the deduced protein sequence of OsBIHD1 contains all conserved domains, a homeodomain, a BELL domain, a SKY box, and a VSLTLGL box, which are characteristics of the BELL type homedomain proteins. The recombinant OsBIHD1 protein expressed in Escherichia coli bound to the TGTCA motif that is the characteristic cis-element DNA sequence of the homeodomain transcriptional factors. Subcellular localization analysis revealed that the OsBIHD1 protein localized in the nucleus of the plant cells. The OsBIHD1 gene was mapped to chromosome 3 of the rice genome and is a single-copy gene with four exons and three introns. Northern blot analysis showed that expression of OsBIHD1 was activated upon treatment with benzothiadiazole (BTH), which is capable of inducing disease resistance. Expression of OsBIHD1 was also up-regulated rapidly during the first 6 h after inoculation with Magnaporthe grisea in BTH-treated rice seedlings and during the incompatible interaction between M. grisea and a resistant genotype. These results suggest that OsBIHD1 is a BELL type of homeodomain transcription factor present in the nucleus, whose induction is associated with resistance response in rice.

Key words

Benzothiadiazole (BTH) - rice (Oryza sativa L.) - homeodomain; disease resistance response - OsBIHD1 - Magnaporthe grisea.

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F. Song

Department of Plant Protection
College of Agriculture and Biotechnology
Zhejiang University - Huajiachi Campus

Hangzhou

Zhejiang 310029

P.R. China

Email: fmsong@zju.edu.cn

Editor: C. M. J. Pieterse

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