Plant Biol (Stuttg) 2005; 7(5): 516-525
DOI: 10.1055/s-2005-865841
Research Paper

Georg Thieme Verlag Stuttgart KG · New York

Results on Quantitative Trait Loci for Flushing Date in Oaks Can Be Transferred to Different Segregating Progenies

O. Gailing1 , A. Kremer2 , W. Steiner3 , H. H. Hattemer1 , R. Finkeldey1
  • 1Institute of Forest Genetics and Forest Tree Breeding, Georg August University Göttingen, Büsgenweg 2, 37077 Göttingen, Germany
  • 2INRA UMR Biodiversité Gènes et Ecosystèmes, 33612 Cestas Cedex, France
  • 3Niedersächsische Forstliche Versuchsanstalt, Waldgenressourcen, Forstamtsstraße 6, 34355 Staufenberg, Germany
Further Information

Publication History

Received: March 16, 2005

Accepted: May 24, 2005

Publication Date:
15 September 2005 (online)


Flushing date (bud burst) is one of the most important traits for the adaptation to different environments and climates in the temperate zone. Because of their wide geographic distribution, Quercus robur L. and Q. petraea (Matt.) Liebl. are suitable as model plants to study the genetic basis of bud burst. QTLs (Quantitative Trait Loci) with comparatively large effects have been mapped in a former study in a Q. robur × Q. robur full-sib family (French cross). In the present study, we performed a Bulked Segregant Analysis (BSA) in the F1 progeny comprising 144 seedlings derived from a cross between a single Q. robur tree as common seed parent and five different pollen donors both from Q. robur and Q. petraea (Q. robur × Q. spp., Diekholzen crosses). In addition, markers linked to two bud burst QTLs with comparably strong effect in the above-mentioned full-sib family (French cross) were tested for their association with bud burst in the Q. robur × Q. spp. (Diekholzen) progeny. Using three microsatellite markers as anchor points, we could map QTLs on linkage group 7 and on linkage group 2, together explaining 16.2 % of the total phenotypic variance (PVE) in 1999 and 38.1 % in 2003. Out of 10 markers that segregated in both mapping progenies, four markers including the two microsatellite markers, showed a significant effect on bud burst in both materials. At microsatellite loci ssrQpZAG1/5 (linkage group 7) and ssrQpZAG119 (linkage group 2) alleles associated with early (allele 166 bp in ssrQpZAG1/5) and late bud burst (allele 57 bp in ssrQpZAG119) in the Q. robur × Q. robur full-sib family (French cross) showed a highly significant association with the same polarity of the effect in the Q. robur × Q. spp. (Diekholzen) progeny. The usefulness of these markers for marker-assisted selection in full-sib and half-sib families is discussed.


O. Gailing

Institute of Forest Genetics and Forest Tree Breeding
Georg August University Göttingen

Büsgenweg 2

37077 Göttingen



Editor: M. Koornneef