Exp Clin Endocrinol Diabetes 2005; 113(8): 423-429
DOI: 10.1055/s-2005-865803
Article

J. A. Barth Verlag in Georg Thieme Verlag KG Stuttgart · New York

The Metabolisable Hexoses D-Glucose and D-Mannose Enhance the Expression of IRS-2 but not of IRS-1 in Pancreatic β-Cells

I. Amacker-Françoys[*] 1 , S. Mohanty[*] 1 , M. Niessen1 , G. A. Spinas1 , T. Trüb2
  • 1Division of Endocrinology and Diabetes, University Hospital, Zurich, Switzerland
  • 2Department for Equipment and Logistics, University Zürich, Zürich, Switzerland
Further Information

Publication History

Received: May 2, 2005 First decision: May 29, 2005

Accepted: June 5, 2005

Publication Date:
08 September 2005 (online)

Abstract

D-glucose regulates maintenance and function of pancreatic β-cells. Several studies have shown that IRS-2, but not IRS-1, is necessary to maintain and sufficient to expand functional β-cell mass. We therefore analyzed the expression of IRS-2 and IRS-1 in β-cells after culture in the presence of various concentrations of D-glucose and other metabolisable or non-metabolisable hexoses. D-glucose increased Irs-2 transcription and IRS-2 accumulation in a dose-dependent manner (1.6 to 25 mmol/l), with a 3-fold increased plateau after 10 h. In contrast, the expression of IRS-1 remained unaffected. D-glucose also induced phosphorylation of IRS-2 while non-metabolisable hexoses did neither affect expression nor phosphorylation. D-glucose-mediated elevation and phosphorylation of IRS-2 were independent of autocrine insulin action although insulin itself could transiently and slightly enhance IRS-2 expression.

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1 Authors contributed equally to this work.

Markus Niessen

Division of Endocrinology and Diabetes
University Hospital

Zurich

Switzerland

Phone: + 41442552225

Fax: + 41 4 42 55 97 41

Email: markus.niessen@usz.ch

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