Pneumologie 2005; 59 - P334
DOI: 10.1055/s-2005-864291

Impact of different lipid emulsions on acute lung injury in a murine model

MH Bi 1, BE Wang 2, J Ott 1, A Mohr 1, MB Schäfer 1, W Seeger 1, K Mayer 1
  • 1Medizinische Klinik II, Klinikum der Justus Liebig Universität Gießen, University of Gießen Lung Center
  • 2Frienship Hospital, Bejing, China

Background: Acute lung injury (ALI) is the most common presentation of sepsis-associated multiple organ failure, characterized by increased alveolar cytokines and inflammatory cell infiltration. Arachidonic acid gives rise to the eicosanoid family of inflammatory mediators and through these regulates activities of inflammatory cells and production of cytokines. Lipid emulsions are used as supplement of parenteral nutrition in intensive care units. Clinoleic®, a new lipid emulsion, was developed to reduce arachidonic acid available, which might reduce inflammatory responses. We investigated impact of two different lipid emulsions on acute lung injury LPS-induced a murine model.

Method: Mice were infused for three days with Clinoleic®, Lipoven®, or NaCl, and were sacrificed at 4h, 8h, 24h after intratracheal LPS-injection. TNF-αand MIP-2 in bronchoalveolar lavage fluid (BAL) and plasma were evaluated by ELISA. Plasma free fatty acids were determined by gas chromatography. Leukocytes in BAL were counted under light microscope.

Results: Clinoleic significantly reduced free arachidonic acid compared to Lipoven by 30%. Significant increases in TNF-α and MIP-2 in BAL were observed at 4h and 8h, decreasing at 24h to baseline after LPS challenge in all groups. There was no significant difference of cytokines in BAL between both lipid emulsion and NaCl groups. Plasma cytokines were much lower than those of in BAL. LPS induced a markedly higher level of leucocytes counts in BAL in both lipid emulsions and NaCl groups, while significantly increasing in Lipoven group at peaking 24h compared to the NaCl group.

Conclusion: In our murine model of ALI, Clinoleic reduced free arachidonic acid. However, cytokine responses didn't differ between the groups. Although LPS caused increasing alveolar leucocytes invasion in both lipid and NaCl groups, Lipoven induced to a higher extent. We speculate that by increasing arachidonic acid, Lipoven induced higher leukocytes transmigration.