Intrauterine Cerebral Processing of External Vibroacoustic Stimuli

The human and the sheep fetus, the animal model of human pregnancy, respond to external stimuli such as vibrations or noises with behavior state changes from 0.8 gestation onwards. It is not clear yet 1) if the behavior state changes that represent a „subcortical“ arousal are accompanied by a „cortical“ arousal as a condition of conscious perception of the stimuli; 2) to what extent the perception of the stimuli depends on the behavior state and the quality of the stimulus; and 3) if the proprioceptive system is important for perception of the vibratory component of the signal. Via Cesarean section, sheep fetuses were chronically instrumented at 0.8 gestation with ECoG (electrocorticogram) electrodes as well as EOG, EMG and EKG electrodes to define fetal behavior states. After a recovery period of five days, stimuli were presented from the abdominal wall of the ewe during periods of NREM and REM sleep. We tested pure vibratory (30min) and acoustic (5h) stimuli as well as dynamic amplitude and frequency modulated and two static vibroacoustic stimuli (VAS) produced by an electronic artificial larynx (EAL) and a laboratory shaker. ECoG changes were analyzed using linear (power spectral analysis) and non-linear methods. For non-linear analysis we used an algorithm based on the Wolf algorithm (calculation of the leading Lyapunov exponent) which calculates a point prediction error (PE) regarding the course of the time series in the phase space. The vibratory and acoustic stimuli did not induce a cortical arousal. The VAS produced an arousal with similar effectiveness during NREM and REM sleep. The dynamic stimulus induced 100%, the EAL 70% and the laboratory shaker 40–50% responses. The PE showed a high predictability in NREM and a low predictability of the ECoG in REM sleep. Although the power spectrum differed between VAS in NREM (decrease of delta activity) and REM sleep (beta activation), the PE showed that the cortical activation pattern did not depend on the sleep state and the stimulus. Processes of cortical activation and deactivation during spontaneous sleep state changes and VAS were similar in their time courses. Cochlear ablation prevented from arousal, suggesting that stimuli were detected through the auditory rather than the proprioceptive system. The results show that external VAS induce a cortical arousal during the last trimester depending on the stimulus. The time course of cortical activation within 32s suggests an arousal via the ascending activating reticular system rather than via specific fiber systems.