Alterations of redox-homeostasis in bowel parts

Introduction: Unhealthy nutritional costumes could result in inflammatory processes and necrosis in the gastrointestinal tract in which free radical reactions are involved.

Aim: Now, the question is, whether the lipid rich diet is able to influence on cellular redox state in all parts of gut in alimentary induced bowel disease.

Methods: Twenty Wistar rats were divided into two groups: control and fat rich diet fed groups. Lipid rich diet (2% cholesterol 0.5% cholic acid, 20% sunflower oil added to the rat chow) was applied for 10 days. After exsanguinations in deep pentobarbital narcosis, bowel parts were isolated and prepared. The redox-status of mucosal homogenates of duodenum, jejunum, ileum, coecum, colon and rectum was determined by H-donating ability (HAD) (Hatano 1988), reducing power (RP) (Oyaizu 1986) and tissue chemiluminescent intensity measurements (TSC) (Blázovics 1999). The biochemical changes were controlled by histological examinations. (Permission number: MÁB1.81/8/1999)

Results and discussion: The small intestine and duodenum are the second commonest sites of polyp development after colon. Polyposis is associated with a high frequency gastrointestinal cancer. The redox homeostasis is very important for the equilibrium among cancer develop, apoptosis, regeneration and necrosis.

RP was very high in duodenum of fat rich diet fed animals, but the data were not changed in jejunum, ileum, coecum, colon and rectum. HAD was better in colon of normolipidemic animals and ileum of hyperlipidemic ones. TSC was very wrong in all parts of short and large bowel and rectum in hyperlipidemia, but the differences were enormous between upper and lower parts compared to the control ones. The total antioxidative status of doudenum, jejunum and ileum were injured less than coecum, colon and rectum. ETT 002/2003.