The histochemical distribution of capsaicin receptors, substance P and calcitonin gene related peptide in the indomethacin treated rats without and with administration of omeprazol

Aims: It is well known that the indomethacin produced gastric mucosal damage in the fundus during 4 hours period. Earlier the possible role of vascular permeability has been studied in this model (Mózsik et al.: Exp. Clin Gastroenterol. 3: 2O5–215, 1993). Recently the possible preventive role of capsaicin was indicated against the indomethacin-induced gastric mucosal damage in rats. (Mózsik, Abdel-Salam, Szolcsányi: Capsaicin-Sensitive Afferent Nerves in the Gastric mucosal Damage and Protection, Akadémiai Kiadó, Budapest, 1997).

Aim of this study was to detect the immundistribution of CR, CGRP and SP in the rat fundus during indomethacin –induced gastric mucosal damage without and with administration of omeprazole.

Materials and methods: CFY strain rats (180–210g) and fasted 24 hours before the experiment. The animals were treated with 20mg / kg indomethacin (Sc). The one group of animals received only saline solution, meanwhile the other omeprazole (5, 10mg/kg) at the same time as IND. The animals were sacrificed 4 hours after IND administration. The number and severity of gastric mucosal damage were detected. The Immmunostaining of CR, CGRP and SP was detected by mono- polyclonal antibodies (Abcam, Cambridge, UK).

Results: 1. Ind.-induced was decreased doses dependently by omeprazole; 2. the CR, CGRP and SP were decreased after omeprazole administration.

Conclusion: The results indicate the involvement of capsaicin afferent nerves in the development of IND. induced gastric mucosal damage and omeprazole protection. The study was supported by the grant of Hungarian Research Found (OTKA – T 047126).