3,5-diiodothyronine Mimics the Effect of Triiodothyronine on Insulin-like Growth Factor Binding Protein-4 Expression in Cultured Rat Hepatocytes

Abstract

We have previously demonstrated that triiodothyronine (T₃) stimulates hepatic IGFBP-4 expression in rats. Since there is evidence that some of the genes whose expression is regulated by T₃ are also sensitive to 3,5-diiodothyronine (T₂), we used the adult rat hepatocyte model in primary cultures directly exposed to T₂ to evaluate insulin-like growth factor binding protein-4 (IGFBP-4) expression by Northern and Ligand blot analyses in this study. Our results demonstrate that T₂, like T₃, is able to enhance IGFBP-4 mRNA and protein after 12 - 24 h of incubation. The potency of the two iodothyronines is comparable as judged by dose-dependence experiments. The T₂-induced IGFBP-4 increase is independent from ongoing protein synthesis but dependent on active transcription. Since T₃ and T₂ do not affect IGF-I production, it appears that the iodothyronines affect the hepatic IGF system at the IGFBP level. Our data, demonstrating that T₂ mimics the stimulatory effect of T₃ on IGFBP-4 expression by rat hepatocytes, allow us to include IGFBP-4 gene among the genes regulated by the two iodothyronines.

Key words

Thyroid hormone - IGF - Liver

References

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