Horm Metab Res 2004; 36(9): 625-629
DOI: 10.1055/s-2004-825927
Original Clinical
© Georg Thieme Verlag KG Stuttgart · New York

Absence of Anorectic Effect to Acute Peripheral Leptin Treatment in Adult Rats whose Mothers Were Malnourished during Lactation

M.  C.  F.  Passos1 , L.  L.  Vicente2 , P.  C.  Lisboa2 , E.  G.  de Moura2
  • 1Department of Applied Nutrition, Nutrition Institute, State University of Rio de Janeiro, Rio de Janeiro, Brazil
  • 2Department of Physiological Sciences, Roberto Alcantara Gomes Biology Institute, State University of Rio de Janeiro, Brazil
Further Information

Publication History

Received 14 January 2004

Accepted after revision 24 May 2004

Publication Date:
15 October 2004 (online)


Diets with restricted energy or protein during lactation programs body weight in the adult offspring. We have investigated the hypothesis that protein or energy-restricted diets during lactation alter the feeding response to peripheral leptin treatment of the adult offspring. Five Wistar rats were randomly assigned to one of the following groups on the day that the offspring were born: C, control diet with 23 % protein; PR, protein restricted diet with 8 % protein; and ER, energy-restricted, receiving the control diet in restricted quantities, which were calculated according to the mean ingestion of the PR group. After weaning (day 21), two animals from each litter (10 pups in each group) were randomly selected and placed together in the cage with free access to water and standard diet until 150 days of age, when they were tested for its response to either leptin (0.5 mg/kg body wt ip) for groups Clep, PRlep and ERlep or saline vehicle for groups Csal, PRsal and ERsal on food intake. In the control groups, food intake was reduced two hours (36 %), four hours (41 %) and six hours (25 %) after leptin treatment. In contrast, no response was observed to leptin treatment in the PRlep and ERlep groups, suggesting leptin resistance. We demonstrated the development of resistance to the anorectic leptin effect and its program in a critical life period associated to nutritional and hormonal factors.


Dr. E. G. de Moura

Departamento de Ciências Fisiológicas, 5o andar, Instituto de Biologia, Universidade do Estado do Rio de Janeiro

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