Horm Metab Res 2004; 36(9): 630-638
DOI: 10.1055/s-2004-825904
Original Clinical
© Georg Thieme Verlag KG Stuttgart · New York

Reduction of Postprandial Hyperglycemia in Patients with Type 2 Diabetes Reduces NF-κB Activation in PBMCs

G.  Rudofsky, Jr.1 , P.  Reismann1 , S.  Schiekofer1 , D.  Petrov1 , M.  von  Eynatten1 , P.  M.  Humpert1 , B.  Isermann1 , C.  Müller-Hoff1 , T.-P.  Thai1 , S.  Lichtenstein1 , U.  Bärtsch1 , A.  Hamann1 , P.  Nawroth1 , A.  Bierhaus1
  • 1Department of Medicine I, University of Heidelberg, Germany
Further Information

Publication History

Received 5 January 2004

Accepted after revision 24 March 2004

Publication Date:
05 October 2004 (online)

Abstract

Aims/hypothesis: Short-lasting hyperglycemia results in activation of the transcription factor NF-κB in peripheral blood mononuclear cells. We therefore studied whether the postprandial increase in glucose is sufficient to induce mononuclear NF-κB activation and whether blunting postprandial hyperglycemia with the alpha-glucosidase inhibitor acarbose reduces NF-κB activation.

Methods: 20 patients with type 2 diabetes were included in a double-blind randomized trial receiving 100 mg acarbose or placebo three times a day over a period of eight weeks. Peripheral blood mononuclear cells were isolated before and 120 minutes after a standardized breakfast. NF-κB binding activity was estimated by electrophoretic mobility shift assay and NF-κB-p65; translocation was determined by Western blot.

Results: Eight weeks of treatment with acarbose significantly reduced postprandial hyperglycemia (p = 0.004 when compared to placebo), postprandial mononuclear NF-κB-binding activity (p = 0.045) and nuclear translocation of NF-κB-p65 (p = 0.02).

Conclusion: Reduction of postprandial glucose peak levels by acarbose reduces postprandial mononuclear NF-κB activation.

References

Angelika Bierhaus, Ph.D.

Medizinische Klinik I der Universität Heidelberg, Otto-Meyerhof-Zentrum ·

Im Neuenheimer Feld 350 · 69120 Heidelberg · Germany

Phone: +49 (6221) 564752

Fax: +49 (6221) 564754 ·

Email: angelika_bierhaus@med.uni-heidelberg.de