Isolation and Characterization of Mouse Hepatic Stem Cells in Vitro

Mary C. Weiss; Hélène Strick-Marchand

doi:10.1055/s-2004-815558

Seminars in Liver Disease, Table of Contents

Semin Liver Dis 2003; 23(4): 313-324
DOI: 10.1055/s-2004-815558

Isolation and Characterization of Mouse Hepatic Stem Cells in Vitro

Mary C. Weiss^1,2 , Hélène Strick-Marchand²

¹Professor, Departement de Biologie de Developpement, Institut Pasteur, Paris, France
²Departement de Biologie de Developpement, Institut Pasteur, Paris, France

Abstract

ABSTRACT

This article covers mouse cell lines showing the properties of hepatoblasts or oval cells and describes hepatocyte differentiation obtained from embryonic stem (ES) cells and mesenchymal adult progenitor cells. It considers the methods used for cultivation and differentiation of the cells, as well as the strengths and weaknesses of the different systems and perspectives for future research. Emphasis is given to hepatoblast cell lines because this is the material for which bipotentiality has been most clearly demonstrated and for which proliferation and differentiation conditions have been worked out.

KEYWORDS

Hepatoblasts - oval cells - hepatocytes - cholangiocytes

Full Text

References

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