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The serotonergic receptor 2C subtype (5-HT2C) regulates survival and proliferation of adult neural stem cells (ANSC) derived from mouse hippocampus
To study the impact of serotonin on neurogenesis, we established an in-vitro model of primary cultures from adult mouse hippocampi. We were able to show that these cells produce serotonin and express key proteins of the serotonergic system.
PCR results were positive for the 5-HT2C receptor. In addition we functionally knocked-out the 5-HT2C receptor by the SB206553 compound. ANSC treated with SB206553 at a concentration of 150uM showed a significant (p<0.05) reduction in vitality of 30% compared to non-treated stem cells.
This is the first report on the presence of serotonin-related proteins on ANSC and their functional role. The results underpin the influence of serotonin and its elements on the complex process of neurogenesis. In particular the 5-HT2C receptor subtype warrants further investigation.