Planta Med 2003; 69(12): 1169-1171
DOI: 10.1055/s-2003-818014
© Georg Thieme Verlag Stuttgart · New York

Quantitative Analysis of Strychnine and Brucine in Strychnos nux-vomica using 1H-NMR

Michel Frédérich1 , 2 , Young Hae Choi1 , Robert Verpoorte1
  • 1Division of Pharmacognosy, Leiden University, Institute of Biology, Section Metabolomics, Leiden, The Netherlands
  • 2Laboratory of Pharmacognosy, Natural and Synthetic Drug Research Center, University of Liège, Liège, Belgium
The Post-doctoral Fellowship Program of the Korea Science Engineering Foundation (KOSEF) and the Belgian National Fund for Scientific Research (FNRS) are gratefully acknowledged for their support to of Y.H. Choi and M. Frédérich, respectively
Further Information

Publication History

Received: May 7, 2003

Accepted: September 7, 2003

Publication Date:
29 January 2004 (online)


A quantitative analysis using 1H-NMR (Q-NMR) has been developed for the determination of strychnine and brucine in Strychnos nux-vomica seeds and stems. The advantages of the method are that no reference alkaloids are needed for calibration curves, the quantification could be directly realized on a crude extract, strychnine and brucine could easily be distinguished, an overall profile of the preparation (including non alkaloid compounds) could be directly obtained, and a very significant time-gain could be achieved, in comparison to conventional HPLC methods, for instance.


Robert Verpoorte

Division of Pharmacognosy

Leiden University

Institute of Biology

Section Metabolomics

P.O. Box 9502

2300 RA Leiden

The Netherlands

Fax: +31-71-527-4503