Planta Med 2003; 69(8): 696-700
DOI: 10.1055/s-2003-42790
Original Paper
© Georg Thieme Verlag Stuttgart · New York

Suppression of Inducible Nitric Oxide Production by Indole and Isothiocyanate Derivatives from Brassica Plants in Stimulated Macrophages

Yue-Hwa Chen1 , Huey-Jing Dai1 , Hsiao-Pei Chang1
  • 1Taipei Medical University, Graduate Institute of Nutrition and Health Sciences, Taipei, Taiwan, R.O.C.
This work was supported by a grant from the National Science Council (NSC 89-2320-B-038-053), Taiwan, ROC
Further Information

Publication History

Received: December 18, 2002

Accepted: April 21, 2003

Publication Date:
06 October 2003 (online)


In this study, the effects of bioactive compounds derived from vegetables of the Brassica genus (Brassicaceae) including 2-phenylethyl isothiocyanate (PEITC), indole-3-carbinol (I3C), and indolo[3,2-b]carbazole (ICZ), on the inhibition of NO production in RAW 264.7 cells were explored. The results indicated that PEITC and I3C inhibited lipopolysaccharide (LPS)- and interferon-γ (IFN-γ)-induced NO production in RAW 264.7 cells, and this inhibition was in accordance with lowering the expression of iNOS protein and mRNA. On the contrary, ICZ, a derivative of I3C, had no significant effect on the stimulated NO production. In conclusion, the Brassica plants derivatives, PEITC and, to a lesser extent, I3C inhibit the LPS/IFN-γ-induced NO production by lowering iNOS protein and mRNA expression in RAW 264.7 cells, in which the PEITC had a more potent inhibitory effect. Nevertheless, ICZ exhibits no inhibitory effect on the activated NO production (Indole-3-carbinol = indole-3-methanol).


Dr. Yue-Hwa Chen

Taipei Medical University

Graduate Institute of Nutrition and Health Sciences

250 Wu-Hsing Street

Taipei 110


Republic of China

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