Abstract
In this study, the effects of bioactive compounds derived from vegetables of the Brassica genus (Brassicaceae) including 2-phenylethyl isothiocyanate (PEITC), indole-3-carbinol
(I3C), and indolo[3,2-b]carbazole (ICZ), on the inhibition of NO production in RAW 264.7 cells were explored.
The results indicated that PEITC and I3C inhibited lipopolysaccharide (LPS)- and interferon-γ
(IFN-γ)-induced NO production in RAW 264.7 cells, and this inhibition was in accordance
with lowering the expression of iNOS protein and mRNA. On the contrary, ICZ, a derivative
of I3C, had no significant effect on the stimulated NO production. In conclusion,
the Brassica plants derivatives, PEITC and, to a lesser extent, I3C inhibit the LPS/IFN-γ-induced
NO production by lowering iNOS protein and mRNA expression in RAW 264.7 cells, in
which the PEITC had a more potent inhibitory effect. Nevertheless, ICZ exhibits no
inhibitory effect on the activated NO production (Indole-3-carbinol = indole-3-methanol).
Key words
Brassicaceae
- indole - isothiocyanate - nitric oxide - inducible nitric oxide synthase - RAW 264.7
macrophages
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Dr. Yue-Hwa Chen
Taipei Medical University
Graduate Institute of Nutrition and Health Sciences
250 Wu-Hsing Street
Taipei 110
Taiwan
Republic of China
Telefon: +886-2-27361661 ext. 6555 ext. 118
Fax: +886-2-27373112
eMail: yuehwa@tmu.edu.tw
