Planta Med 2003; 69(8): 710-714
DOI: 10.1055/s-2003-42787
Original Paper
Pharmacology
© Georg Thieme Verlag Stuttgart · New York

Anti-Inflammatory Effect of the Oligomeric Stilbene α-Viniferin and its Mode of the Action through Inhibition of Cyclooxygenase-2 and Inducible Nitric Oxide Synthase

Eun Yong Chung1 , Byung Hak Kim1 , Myung Koo Lee1 , Yeo-Pyo Yun1 , Seung Ho Lee2 , Kyung Rak Min1 , Youngsoo Kim1
  • 1College of Pharmacy and Research Center for Bioresource and Health, Chungbuk National University, Cheongju, Korea
  • 2College of Pharmacy, Yeungnam University, Kyeongsan, Korea
This work was financially supported by a grant (KRF-99-042-F00167) from Korea Research Foundation
Weitere Informationen

Publikationsverlauf

Received: November 19, 2002

Accepted: April 13, 2003

Publikationsdatum:
06. Oktober 2003 (online)

Abstract

The anti-inflammatory activity of α-viniferin, a trimer of resveratrol, has been demonstrated in an animal model of carrageenin-induced paw edema, and inhibitory effects of the compound on cyclooxygenase (COX) and inducible nitric oxide synthase (iNOS) have been investigated in order to understand the mode of the observed action. α-Viniferin at doses > 30 mg/kg (p. o.) or > 3 mg/kg (i. v.) showed significant anti-inflammatory activity on carrageenin-induced paw edema in mice. α-Viniferin showed an inhibitory effect with an IC50 value of 4.9 μM on COX-2 activity but a very weak inhibitory effect with 55.2 ± 2.1 % of the control (100 %) at 100 μM on COX-1 activity. α-Viniferin at doses of 3 μM to 10 μM inhibited the synthesis of COX-2 transcript in lipopolysaccharide (LPS)-activated murine macrophages Raw264.7. α-Viniferin showed an IC50 value of 2.7 μM on nitric oxide (NO) production in LPS-activated Raw264.7 cells when α-viniferin and LPS were treated simultaneously, but did not inhibit the NO production when α-viniferin was treated at 12 h after LPS stimulation. α-Viniferin inhibited synthesis of iNOS transcript with an IC50 value of 4.7 μM. Consequently, the inhibitory effect of α-viniferin on the release of prostanoids and NO could play an important role to show anti-inflammatory action.

References

Prof. Youngsoo Kim, Ph. D.

College of Pharmacy

Chungbuk National University

Cheongju 361-763

Korea

Fax: +82-43-268-2732

eMail: youngsoo@cbucc.chungbuk.ac.kr