Planta Med 2003; 69(8): 739-744
DOI: 10.1055/s-2003-42785
Original Paper
Biochemistry, Physiology, in vitro-Cultures
© Georg Thieme Verlag Stuttgart · New York

The Bioconversion Process of Deoxypodophyllotoxin with Linum flavum Cell Cultures

Albert Koulman1 , Aäron C. Beekman1 , 2 , Niesko Pras1 , Wim J. Quax1
  • 1Department of Pharmaceutical Biology, GUIDE (Groningen University Institute for Drug Exploration), University of Groningen, Groningen, The Netherlands
  • 2Current address: Department of Molecular Genetics, University of Groningen, Haren, The Netherlands
Further Information

Publication History

Received: December 20, 2002

Accepted: April 12, 2003

Publication Date:
06 October 2003 (online)

Abstract

The in vitro cell suspension culture of Linum flavum is able to convert high amounts of the 2,7′-cyclolignan deoxypodophyllotoxin into 6-methoxypodophyllotoxin 7-O-glucoside. We studied this conversion in detail by monitoring the intermediates and side-products after feeding different concentrations of deoxypodophyllotoxin. At a low concentration (0.1 mM) deoxypodophyllotoxin is rapidly converted into 6-methoxypodophyllotoxin 7-O-glucoside, 6-methoxypodophyllotoxin and traces of β-peltatin and podophyllotoxin. The feeding of 0.5 and 2.0 mM also shows a rapid conversion into 6-methoxypodophyllotoxin 7-O-glucoside, but a delayed formation of 6-methoxypodophyllotoxin and β-peltatin. By using different extraction methods we delivered proof in favour of the hypothesis that a part of the deoxypodophyllotoxin after uptake is temporarily stored as β-peltatin glucoside.

References

Albert Koulman

Department of Pharmaceutical Biology

GUIDE (Groningen University Institute for Drug Exploration)

University of Groningen

A. Deusinglaan 1

9713 AV Groningen

The Netherlands

Phone: +031-50-36333363

Fax: +031-50-3633000

Email: A.Koulman@farm.rug.nl