Planta Med 2003; 69(8): 778-779
DOI: 10.1055/s-2003-42781
© Georg Thieme Verlag Stuttgart · New York

In Vitro Micropropagation, Differentiation of Aerial Bulbils and Tubers and Diosgenin Content in Dioscorea bulbifera

Alka Narula1 , Sanjeev Kumar2 , K. C. Bansal3 , P. S. Srivastava1
  • 1Centre for Biotechnology, Jamia Hamdard, New Delhi, India
  • 2Amity Institute of Biotechnology, Amity IT University, Noida, UP, India
  • 3National Research Centre on Plant Biotechnology, I A R I, New Delhi, India
Further Information

Publication History

Received: December 18, 2002

Accepted: March 18, 2003

Publication Date:
06 October 2003 (online)


Dioscorea bulbifera could be micropropagated through nodal segments and bulbils. The best medium for regeneration and bulbil differentiation was MS + 0.5 μM IAA (indole-3-acetic acid) + 20.0 μM Kn (kinetin) + 500 mg/L CH (casein hydrolysate) + activated charcoal (20 %). Diosgenin content was maximum in regenerants grown on MS + 5.0 μM IAA + 20.0 μM Kn + 500 mg/L CH. T.s of bulbils could also be used for direct plantlet differentiation as well as bulbil differentiation on MS + 10.0 μM IAA + 20.0 μM Kn + (in mg/L) 30 each of Asp (asparagine) + Arg (arginine) + Gln (glutamine) + 10 Ad (adenine) + 500 CH + 10 Cyst hyd (cysteine hydrochloride). Diosgenin yield in plantlets reached a maximum after 20 weeks. The results indicate that micropropagation, bulbil formation and tuberisation can be achieved in vitro in D. bulbifera, hitherto a less exploited plant, and can further be used for obtaining enhanced levels of diosgenin.


Professor P S Srivastava

Centre for Biotechnology

Faculty of Science

Jamia Hamdard

New Delhi 110062


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